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. 2020 Jun 11;10:865. doi: 10.3389/fonc.2020.00865

Figure 6.

Figure 6

Wnt/β-catenin signaling pathway inhibitor XAV939 eliminated the disadvantages aroused by UCHL5 overexpression in AN3-CA cells. (A) UCHL5 overexpression (oeUCHL5). AN3-CA cells were treated with or without Wnt/β-catenin signaling pathway inhibitor XAV939 (10 μM) for 24 h. Relative protein levels of β-catenin were detected by WB. *P < 0.05 vs. empty vector; #P < 0.05 vs. oeUCHL5; &P < 0.05 vs. vector + XAV939. (B,C) Relative protein levels of CyclinD1, C-myc, cleaved-caspase3, and Survivin were detected by WB. *P < 0.05 vs. empty vector; #P < 0.05 vs. oeUCHL5; &P < 0.05 vs. vector + XAV939. (D) Cell cycle was measured by Cell Cycle Analysis Kit (N ≥ 3). *P < 0.05 vs. empty vector; #P < 0.05 vs. oeUCHL5; &P < 0.05 vs. vector + XAV939. (E) Relative cell viability were detected by CCK-8 at different time points (N = 3). *P < 0.05 vs. empty vector; #P < 0.05 vs. oeUCHL5; &P < 0.05 vs. vector + XAV939. (F) The percentage of Apoptosis cells (N ≥ 3). *P < 0.05 vs. empty vector; #P < 0.05 vs. oeUCHL5; &P < 0.05 vs. vector + XAV939.