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. 2020 Jun 11;11:825. doi: 10.3389/fphar.2020.00825

Table 1.

The primers.

Gene Primer sequence (5′->3′) Product size (bp) Annealing temperature (°C)
GAPDH F: CAATGACCCCTTCATTGACC
P: GACAAGCTTCCCGTTCTCAG
106 59/60
Notch1 F: ACCAATACAACCCTCTGCGG
P: GGCCCTGGTAGCTCATCATC
141 59
β-catenin F: ATGAC TCGAGCTCAGAGGGT
P:ATTGCACGTGTGGCAAGTTC
99 60
C-myc F:CGTCCTCGGATTCTCTGCTC
P:GCTGCGTAGTTGTGCTGATG
186 60