Table 1.
Gene | Primer sequence (5′->3′) | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|
GAPDH | F: CAATGACCCCTTCATTGACC P: GACAAGCTTCCCGTTCTCAG |
106 | 59/60 |
Notch1 | F: ACCAATACAACCCTCTGCGG P: GGCCCTGGTAGCTCATCATC |
141 | 59 |
β-catenin | F: ATGAC TCGAGCTCAGAGGGT P:ATTGCACGTGTGGCAAGTTC |
99 | 60 |
C-myc | F:CGTCCTCGGATTCTCTGCTC P:GCTGCGTAGTTGTGCTGATG |
186 | 60 |