FIGURE 2.

Map-based cloning of the OsPLS4 gene. (A) Fine mapping of PLS4 further localized the mutation to a 52-kb genomic region between C4 and C7 on chromosome 4. The number of recombination events between marker and pls4 phenotype. The target region contains 7 candidate ORFs. (B) Schematic of PLS4. The black rectangles represent exons, and the position of the SNP is indicated in the ninth exon of LOC_Os04g30760; the SNP resulted in the mutation of the encoded protein at position 254 from alanine (Ala) to threonine (Thr). (C) The phenotype of the flag leaves in the transplanted lines, COM-1,-2: two independent complementation plants; Bars = 5 cm. (D) The flag leaf Chl content of the WT, pls4 and two COM independent lines at the heading stage. The data presented are the means ± SDs of three biological replicates. **P < 0.01 (Student’s t-test).