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. 2020 Jun 11;11:782. doi: 10.3389/fpls.2020.00782

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Copyright © 2020 Zhou, Li, Yang, Qu, Ouyang, Jiang, Lin, Zhu, Peng, Fu, Peng, Bian, Tang, Xu and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression patterns and subcellular localization. (A) Expression pattern of OsPLS4. Expression in various organs, including the leaves, roots, culms, leaf sheaths, and panicles at the heading stage in the WT. The data presented are the means ± SDs of three biological replicates. (B) Spatial expression pattern of the OsPLS4 gene in transgenic rice plants harboring the OsPLS4 promoter fused to the GUS gene. (a) germinating seed; (b) spikelet; (c) stem; (d) leaf sheath; (e) tillering leaf; (f) anther. Bars = 1 mm. (C) Subcellular localization of OsPLS4-GFP fusion constructs. The transient expression of OsPLS4-GFP fusion constructs in rice protoplasts and epidermal cells of N. benthamiana leaves was imaged by a confocal microscope. Bars = 5 nm.