Table 1.

Method of MSC harvest and characterization.

References	Source	Cell harvest	Cell treatment	MSC characterization
Khatab et al. (2018)	Human	Heparinised femoral shaft bone marrow aspirate	Cultured in minimal essential medium alpha (α MEM), Fetal Calf Serum (FCS) and Invitrogen to third passage	Trilineage differentiation, Flow cytometry: CD73, CD90, CD105, CD166 +ve
Mao et al. (2018)	Human	Bone marrow aspirate from iliac crest	Cultured in astandard Mesenchymal Stem Cell (MSC) media and changed to chondrogenic media from third passage onwards. miR-92a-3p overexpressed in one group	Trilineage differentiation, Flow cytometry: CD11b, CD19, CD34, CD45, CD73, CD90, CD105, and HLA-DR –ve
Tao et al. (2017)	Human	Synovial membrane tissue	Cultured in standard MSC media to fifth passage. miR-140-5p overexpressed in one group	Trilineage differentiation, Flow cytometry: CD44, CD73, CD90, CD105, CD151 +ve
Wang et al. (2017)	Human	Embryonic stem cell-derived MSCs (obtained from third party)	Cultured in standard MSC media and cells between fourth and seventh passage were utilized	Trilineage differentiation, Flow cytometry: CD73, CD90, CD105 +ve
Wu et al. (2019)	Human	Infrapatellar fat pad obtained following total knee arthroplasty	Cultured in standard MSC media to confluence and used at first passage	Flow Cytometry: CD44, CD73, CD90 +ve. CD34, CD11b, CD19, CD45, HLA-DR present at low levels
Zhang et al. (2016)	Human	Cleavage and blastocyst- stage embryonic stem cells from in vitro fertilization	Cultured in standard MSC media. Further details not stated	Trilineage differentiation, Flow cytometry: CD105, CD24 +ve
Zhang et al. (2018)	Human	Immortalized E1-Myc 16.3 embryonic stem cell-derived MSC	Cultured in standard MSC media and passaged at 80% confluence until use. Grown in defined media for 3 days prior to exosome extraction	Trilineage differentiation, Flow cytometry: CD29, CD44, CD90, CD105 +ve, CD34, CD45, HLA-DR –ve
Zhang et al. (2019)	Human	Immortalized E1-Myc 16.3 embryonic stem cell-derived MSC	Cultured in standard MSC media and passaged at 80% confluence until use. Grown in defined media for 3 days prior to exosome extraction	Trilineage differentiation, Flow cytometry: CD29, CD44, CD90, CD105 +ve, CD34, CD45, HLA-DR –ve
Zhu Y. et al. (2017)	Human	Induced pluripotent stem cell-derived MSC (iMSC) induced from human umbilical cord iPS Synovial MSC (sMSC) from humans undergoing Anterior crucial efforts ligament (ACL) reconstruction	iMSC: iPS cultured for 5 days in mTESR1 (Stemcell) and then cultured in standard MSC media for 2 weeks. The cells were then passaged every 5–7 days until a fibroblastic morphology was adopted sMSC: cultured in standard MSC media, with media changed every 4 days	Trilineage differentiation Flow cytometry: iMSC: CD29, CD44, CD73, CD90 +ve. CD34, CD45, HLA-DR –ve sMSC: CD44, CD73, CD90, CD166 +ve. CD34, CD4, HLA-DR –ve
Jin et al. (2020)	Human	MSCs derived from bone marrow aspirate from the ilium of healthy subjects	Cultured to third to fifth passage with media changed every 48 h	Trilineage differentiation. Flow cytometry: CD29, CD44, CD71 +ve. CD34, CD45, and HLA-DR -ve

^arefers to individual culture condition protocol without addition of stimulating factors.