Figure 5.

Toxicity and Biological activities of modulated fibrils. (A) Various effect on the metabolic activity of SH-SY5Y cells after addition of modulated fibrils. Fibrils were grown in the presence of compounds for 72 h at 37°C and added into the cell culture media. MTT reduction was normalized to control cells. Values represented means ± SEM. (B) Representative time course of disaggregation of fibrils grown in different conditions. Control Aβ fibrils with the addition of 5 µM of L-PGDS (black), Aβ fibrils grown in 250 µM of CPM with the addition of 5 µM of L-PGDS (red), fibrils grown in 250 µM of TRD with the addition of 5 µM of L-PGDS (green). (C) Disaggregation of preformed Aβ(1–40) fibrils by L-PGDS, Aβ fibrils grown in 250 µM of CPM (1:5 peptide:drug ratio) and Aβ fibrils grown in 250 µM of TRD (1:5 peptide:drug ratio). Data shown are means ± SEM (n = 6).