FIG 1.

(Top panel) Bioinformatic identification of potential SPC proteins. The list of 17,713 annotated T. cruzi genes from the Y strain genome assembly was narrowed down to 9,612 genes that do not have orthologues outside the Euglena genus. This list was further trimmed to 4,265 genes by eliminating all genes that have orthologues in Leishmania and T. brucei which lack the SPC. The final filter eliminated genes that did not have orthologues in the monoxenous kinetoplastid parasite Paratrypanosoma confusum, which has an SPC, resulting in a list of 217 potential SPC genes. (Bottom panel) List of seven proteins that have been shown to localize to the SPC. ID, identifier.