FIG 2.

Identification and localization of six SPC proteins. (A) CP4 localizes to the SPC, potentially on the CyQ and MtQ (arrow) microtubule root fibers and the basal bodies associated with the flagellum (arrowhead). (B to D) CP5 (B), CP6 (C), and CP7 (D) localize to the POR of the SPC labeled by the lectin concanavalin A (purple arrows). CP5 also shows basal body localization (arrowhead). (E and F) Two proteins annotated to contain myosin motor domains (MyoF [E] and MyHd [F]) localize to the cytopharynx of the SPC. Nuclei and kinetoplasts in all fluorescent images were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). Scale bars, 2 μM.