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Three additional T. cruzi myosins localize to the SPC. (A) Overexpression and superresolution structured illumination (SR-SIM) microscopy of MyoC-mNeon revealed cytopharynx localization, suggesting that MyoC may provide redundancy to the cytopharynx endocytic machinery. (B and C) MyoB (B) and MyoE (C) both localize to the preoral ridge of the SPC (purple arrows). Nuclei and kinetoplasts in all fluorescent images were stained with DAPI (blue). Scale bars, 2 μM.
