FIG 6.

The C-terminal tail regions of myosin F, C, B, and E are sufficient for SPC targeting. (A) Schematic of myosin genes and the regions (green and red lines) that were fused to the C terminus of mNeon for localization. The green-colored regions were sufficient for localization to the SPC when fused to mNeon, whereas the red-colored regions were not. (B, C, and D) The MyoF-Ct1 (B) and MyoF-Ct3 (D) regions were sufficient to target mNeon to the SPC cytopharynx, although MyoF-Ct3 had a notably less defined localization. MyoF-Ct2 (C) was not sufficient for SPC targeting but did demonstrate localization adjacent to the kinetoplast (arrowhead). (E and F) MyoC-Ct2 (F) was sufficient for targeting mNeon to the SPC cytopharynx, whereas MyoC-Ct1 (E) was not. (G and H) The C-terminal regions of MyoB (G) and MyoE (H) were both sufficient to target mNeon to the SPC preoral ridge. Nuclei and kinetoplasts in all fluorescent images were stained with DAPI (blue). Scale bars, 2 μM.