FIG 7.

Incubations of S. aureus in TSB supplemented with lipid standards. (A) PCA of the lipidomic data indicates that oleic acid and CEs, collectively, have similar effects on the lipid profiles of S. aureus relative to S. aureus grown in neat TSB or TSB with ethanol. (B to E) The oleate and linoleate fatty acids from the oleic acid- and CE-treated S. aureus are readily incorporated into PGs (B), CLs (C), LysylPGs (D), and DGDGs (E), whereas little to no incorporation was observed in the PE- and PC-treated S. aureus. n = 3 per group. Statistics and detailed fatty acid composition from MS/MS experiments can be found in Data Set S1.