Maximum-likelihood virophage major capsid protein (MCP) tree. (a) MCPs from known virophages and from contigs identified in this study were aligned, and a maximum likelihood tree was constructed. The tree was rooted using the Mavirus virophage. Contigs from this study are highlighted in a color corresponding to borough. (b) Genome organization of Brooklyn_3 627, which contains a cysteine protease, minor capsid protein, ATPase, major capsid protein, helicase, and other virophage and predicted genes.