FIGURE 5.

FAM225A facilitates CRC progression via miR‐613/NOTCH3 axis. A and B, HCT116 and SW620 cell lines were co‐transfected with sh‐FAM225A#1 and pcDNA3.1/NOTCH3. Colony formation assay and EdU assay were conducted to test cell proliferation capacity. C, JC‐1 assay was applied to test cell apoptosis ability. D, Apoptosis‐associated protein levels were measured by western blot assay. E, Transwell assay manifested cell migration capability. F, Migration‐related protein and EMT progression‐associated protein levels were determined via western blot. Results were presented as the mean ± SD. **P < .01