FIGURE 5.

FGF2 and CCND2 were direct targets through which miR‐646 regulated proliferation and invasion of NSCLC cells. (A) Predicted binding sites of miR‐646 in the 3′UTR of FGF2 and CCND2. (B) The expression levels of FGF2 and CCND2 mRNA and protein were detected by qRT‐PCR and Western blotting in A549 and SPC‐A1 cells after transfection of miR‐646 mimic or NC mimic. (C) Sequence of miR‐646‐mut. (D) Luciferase activity was analyzed in A549 and SPC‐A1 cells co‐transfected with miR‐646 mimic or miR‐646‐mut mimic with FGF2‐3'UTR or CCND2‐3'UTR reporters. (E,F)The average expression level of FGF2 and CCND2 in NSCLC tissues and adjacent noncancerous lung tissues. (G,H) Pearson's correlation analyses between miR‐646 expression and the expression of FGF2 and CCND2 in NSCLC tissues. *P < .05, **P < .01