FIGURE 2.

Representative images depicting cellular localization of GFP-tagged TDP-43 fragments (donor cells; green) after transfer to full-length td-tomatoTDP43^{1– 414} acceptor cells (acceptor cells; red). Acceptor cells (td-tomatoTDP43^{1– 414}), which also displayed GFP positivity, were sorted and fixed for confocal microscopy. Association of full-length TDP-43 from acceptor cells was observed with varying degrees to each TDP fragment (A–E) as well as with actin (F) and CD63 (G). Construct 10^{1– 105} (D) shows localization exclusively to the nucleus, whereas constructs 2^{86– 414}, 5^{257– 414}, 6^{1– 314}, and WT-TDP-GFP^{1– 414} (A–C,E, respectively) could be found in the cytoplasm, as well as the nucleus. The final column contains Z-projections. Scale bar = 10 μm.