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. 2020 Mar 5;10(2):209–223. doi: 10.1016/j.jcmgh.2020.02.007

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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HNF4α is essential for intestinal organoid growth. (A) Bright-field microscopic images of wild-type and VillinCre-Hnf4a^fl/fl small-intestinal crypts and (B) tamoxifen-induced VillinCreERT2^--Hnf4a^fl/fl and VillinCreERT2⁺-Hnf4a^fl/fl organoids 6 days after tamoxifen induction with and without Wnt3A supplementation. Representative images are shown. Scale bars: 250 μm. (C) Quantification of the amount of organoids in tamoxifen-inducible Hnf4a mutant organoids with and without Wnt3A supplementation at 1 and 7 days after tamoxifen treatment (n = 4). (D) Organoids were passaged 7 days after tamoxifen treatment, and the amount of surviving organoids was quantified on days 1 and 4 after passaging (n = 4). Data are plotted as means ± SEM. ∗∗∗P < .001 as calculated by 2-way analysis of variance. Representative images are shown. W-ENR, epidermal growth factor, Noggin, and R-spondin supplemented with Wnt3A.