Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 5;10(2):209–223. doi: 10.1016/j.jcmgh.2020.02.007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Olfm4 is nearly abolished in Hnf4a-deficient intestinal regeneration. (A) RNAscope in situ hybridization of Lgr5 in VillinCreERT2^--Hnf4a^fl/fl and VillinCreERT2⁺-Hnf4a^fl/fl mice at 96 hours after irradiation in small-intestinal tissue. (B) qRT-PCR of Lgr5 in small-intestinal tissue. (C) RNAscope in situ hybridization of Olfm4 in VillinCreERT2^--Hnf4a^fl/fl and VillinCreERT2⁺-Hnf4a^fl/fl mice at 96 hours after irradiation in small-intestinal tissue. Scale bars: 25 μm. (D) qRT-PCR of Olfm4 in small-intestinal tissue. Gapdh is used as a reference gene. n = 8 and 10 per group. Data are plotted as means ± SEM. ∗∗∗∗P < .0001 as calculated by the Student t test. Slides were counterstained with hematoxylin. Representative images are shown. mRNA, messenger RNA.