Postinflammatory DSS mice exhibit persistent changes in the microbiome. The alpha (A) and beta (B) diversity was assessed in controls, postinflammatory DSS, and mice given antibiotics and then allowed a 2-week washout period. Baseline samples were not different; antibiotic washout allowed some degree of microbial recovery, although there was still a significant difference in the alpha diversity when comparing CT vs CT + Abx groups (Tukey honestly significant difference; P = .0067) and in beta diversity when comparing CT vs CT + Abx groups and DSS vs DSS + Abx groups (permutational multivariate analysis of variance, CT vs CT + Abx, P = .001; DSS vs DSS + Abx, P = .003). Points represent individual samples, lines represent the median, and crosses represent the mean. (C) Comparison of bacterial content at the genus level between control and postinflammatory DSS mice at the washout time point. Blue symbols represent families significantly increased in the post-inflammatory DSS mice, and red symbols represent families significantly increased in controls (beta-binomial regression model, P < .01). N = 6–8/ group. Abx, antibiotics; CT, control; DSS, dextran sulfate sodium.