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Expression of BMP4 and STAR in chicken PHFs. (a) Secretion of BMP4 determined by ELISA. (b) BMP4 mRNA expression determined by qRT-PCR. (c) STAR immunohistochemical staining of D580-SWFs after coculture with D280-SYF-GCs for 72 h. Scale bar: 100 μm (c, left) and 50 μm (c, right). Values were the mean ± SEM of three experiments. Different lowercase letters indicated significant difference (p < 0.05).
