Table 1.
UHPLC retention times (RT) and fragmentation patterns of flavanone catabolites in 24-h urine after consumption of the orange fruit and orange juice test meal
| No. | Compound | RT (min) | [M – H]− (m/z) | MS2 (mlz)a) |
|---|---|---|---|---|
| 1 | 4-Hydroxyhippuric acid (4-OH-HipA) | 1.45 | 194.1 | 150.0, 100.0, 93.1, 74.2 |
| 2 | 3-(3′-Hydroxyphenyl)hydracrylic acid (HPHPA) | 1.90 | 181.1 | 136.9, 121.0, 119.1 |
| 3 | Hippuric acid (HipA) | 2.13 | 178.1 | 134.0, 132.0, 77.1 |
| 4 | 3-(3′-Hydroxy-4′-methoxypheny)propionic acid (HMPPA) | 2.89 | 195.1 | 136.1, 134.9, 119.1, 107.9 |
Hesperetin and naringenin were quantitated by a separate method (see Materials and methods section).
a)
Transitions printed in bold were used as quantifiers for the respective catabolite, whereas the remaining ones were qualifiers to ascertain catabolite identity and peak purity.