Figure 1.

Workflow for pulsed-SILAC in vivo labeling of mouse brain tissues and subsequent proteomic analysis. Male C57BL6/J mice aged 7–8 weeks were acclimatized to their environment for 2–3 weeks on a regular mouse chow diet containing ¹²C₆-l-lysine. After 16 h fasting overnight, the animals were switched onto a ¹³C₆-l-lysine-labeled diet for 2 days duration. The mice were then euthanized, and brain tissues were excised for protein extraction and mass spectrometry analysis. Subsequent proteome analysis allowed the identification of newly synthesized proteins (¹³C₆-l-lysine-labeled) within whole mouse brain tissues. Mouse photograph courtesy of https://imgbin.com/ Copyright 2020.