Figure 2.

Distribution of the average H/L abundance ratio of master protein groups. Brain protein heavy lysine labeling efficiency was determined as 5.6% (based on the average H/L ratio of the three brain proteomes after 2 days pSILAM diet). This value was then used to normalize the abundance ratio, i.e., average H/L of each protein from three biological replicates was divided by 0.056. Among the 4668 protein groups that have been quantified in all the three biological replicates, with LOG2(normalized H/L abundance ratio) adopting a normal distribution, a total of 1418 protein groups displayed H/L ratios less than the proteome-wide average of 0.056, while 3250 protein groups displayed a H/L ratio greater than the average.