TABLE 1.
Summary of Tissue ncRNAs Associated With Various Aspects of Pediatric IBD
| Study | ncRNA | Change in expression (patients) | Compared groups | Number of patients, sample type | Statistical parameters | Technological platform | ||
|---|---|---|---|---|---|---|---|---|
| Best P- achieved | AUC | Sensitivity/ Specificity (%) | ||||||
| Koukos et al., 2013 40 | miR-101 | Down | IBD vs. non-IBD | 45 biopsies | — | — | — | MicroRNA-library screen, RT-qPCR |
| miR-26 | Down | — | ||||||
| miR-124 | Down | pUC vs. pCD/non-IBD | <0.0001/<0.01 | |||||
| Koukos et al., 2015 42 | miR-4284 | Down | pIBD vs. non-IBD | 37 biopsies | <0.05 | — | — | mirCURY microRNA array, RT-qPCR |
| Zahm et al., 2014 43 | miR-192 miR-194 miR-200b miR-375 | Down | pUC vs. controls | 50 biopsies | 0.0006 0.0019 0.0056 0.0001 | — | — | nCounter, TaqMan low density array, RT-qPCR |
| miR-142-3p miR-146a miR-21 let-7i | Up | 0.0048 0.0027 0.0003 0.0007 | — | — | ||||
| miR-24 | — | pUC vs. pCD | — | 0.83 | 83.3/85.7 | |||
| Béres et al., 2016 44 | miR-122 miR-146a miR-155 | Up Up UP | ipCD vs. C/pUC pUC vs. C ipCD vs. C | 28 FF samples, 71 FFPE samples | <0.01 <0.001 <0.001 | — | — | RT-qPCR |
| Szűcs et al., 2016 45 | miR-146a miR-155 | Up | ipCD vs. inpCD vs. C | 30 FFPE samples | <0.001 | — | — | RT-qPCR |
| Béres et al., 2017 33 | miR-185 miR-223 | Up | ipCD vs. C | 44 FF samples | <0.05 <0.001 | 0.81 1 | 62.5/100 100/100 | NGS, RT-qPCR |
| miR-146a miR-142–3p | — | pCD vs. pUC | <0.01 <0.01 | 0.838 0.888 | 80/76.92 77.78/90.31 | |||
| Tang et al., 2018 48 | miR-15a | Down | CDre vs. CDac | 54 FF samples | <0.05 | — | — | RT-qPCR |
Abbreviations: AUC, area under the curve; C, control; FFPE, formalin-fixed paraffin-embedded; CDac, active Crohn’s disease; CDre, Crohn’s disease in remission; FF, fresh frozen tissue; ipCD, histologically intact tissue of pediatric patients with Crohn’s disease; inpCD, histologically inflamed tissue of pediatric patients with Crohn’s disease; NGS, next-generation sequencing; pCD, pediatric patients with Crohn’s disease; pIBD, pediatric patients with inflammatory bowel disease; pUC, pediatric patients with ulcerative colitis; non-IBD, control patients without inflammation typical for IBD; RT-qPCR, real-time quantitative polymerase chain reaction.