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. 2020 Jun 18;9:26. doi: 10.1186/s40035-020-00206-1

Fig. 5.

Fig. 5

CD2AP and BIN1are recruited to early endosome by RIN3. PC12M cells expressing GFP/CD2AP-flag (a, b), RIN3-GFP/CD2AP-flag (c, d), GFP/BIN1-flag (e, f), RIN3-GFP/BIN1-flag (e-h) were immune-stained for Rab5 with a specific antibody. Representative images are shown. Colocalization analysis was performed by imageJ by measuring fluorescence intensity alongside the drawn line. The three different colors peak at the same position If the three proteins were colocalized (b, d, f, h)