| Antibodies |
| pHER2 (Y1248) |
Cell Signaling Technologies |
Cat# 2244 |
| HER2 |
Cell Signaling Technologies |
Cat# 2242 |
| pAKT (S473) |
Cell Signaling Technologies |
Cat# 9271 |
| pAKT (T308) |
Cell Signaling Technologies |
Cat# 13038 |
| pERK |
Cell Signaling Technologies |
Cat# 9101S |
| pS6 (240/4) |
Cell Signaling Technologies |
Cat# 2215S |
| pS6 (235/6) |
Cell Signaling Technologies |
Cat# 2211S |
| pS6K (T389) |
Cell Signaling Technologies |
Cat# 9205 |
| pmTOR (S2448) |
Cell Signaling Technologies |
Cat# 2971S |
|
| Bacterial and Virus Strains |
| One Shot™ MAX Efficiency™ DH5α™-T1 |
Thermo Fisher Scientific |
Cat# 12297016 |
| One Shot™ Stbl3™ Chemically Competent cells |
Thermo Fisher Scientific |
Cat# C737303 |
|
| Biological Samples |
| ST1616B (HER2-amplified, HER2D769Y) breast PDX |
START |
N/A |
| ST1456 (HER2V842I) breast PDX |
START |
N/A |
| HCI003 (HER2G778_P780 dup) breast PDX |
Gift from Dr. Alan Welm, Huntsman Cancer Institute |
N/A |
|
| Chemicals |
| Neratinib (HKI-272) |
PUMA biotechnology / Selleck Chemicals |
Cat# S2150 |
| Everolimus (RAD001) |
Selleck Chemicals |
Cat# S1120 |
| Alpelisib (BYL719) |
Novartis |
N/A |
| Buparlisib (BKM120) |
Selleck Chemicals |
Cat# S2247 |
| MK2206 |
Selleck Chemicals |
Cat# S1078 |
| Afatinib (BIBW2992) |
Selleck Chemicals |
Cat# S7810 |
| Lapatinib |
LC laboratories |
N/A |
| Selumetinib (AZD6244) |
Selleck Chemicals |
Cat# S1008 |
|
|
|
| Critical Commercial Assays |
| Ras Activation Assay Kit |
EMD Millipore |
Cat# 17-218 |
|
| Deposited Data |
| RNA-sequencing |
GEO database |
Accession ID: GSE128730
|
| Whole exome sequencing |
SRA database |
BioProject ID: PRJNA574429 |
|
| Experimental Models: Cell Lines |
| 5637 HER2S310F cell line |
ATCC |
Cat# HTB-9 |
| H1781 HER2
G776>VC cell line |
ATCC |
Cat# CRL-5894 |
| OVCAR8 HER2G776V cell line |
DCDT tumor repository,NCI |
N/A |
| MCF7 HER2V777L cell line |
Gift from Dr. Ben Park, Vanderbilt Ingram Cancer Center |
N/A |
| MCF7 HER2L755S cell line |
Gift from Dr. Ben Park, Vanderbilt Ingram Cancer Center |
N/A |
| DV90 HER2V842I cell line |
DSMZ |
Cat# ACC 307 |
| SNUC2A HER2R678Q cell line |
ATCC |
Cat# CCL-250.1 |
|
| Oligonucleotides |
| Silencer® Select RPTOR siRNA |
Thermo Fisher Scientific |
Assay ID s33216 |
| Silencer® Select RPTOR siRNA |
Thermo Fisher Scientific |
Assay ID s33215 |
| Silencer® Select RHEB siRNA |
Thermo Fisher Scientific |
Assay ID s12019 |
| Silencer® Select HRAS siRNA (validated) |
Thermo Fisher Scientific |
Assay ID: 120898 |
| Silencer® Select KRAS siRNA (validated) |
Thermo Fisher Scientific |
Assay ID 120703 |
| Silencer® Select NRAS siRNA (validated) |
Thermo Fisher Scientific |
Assay ID: 120250 |
|
| Recombinant DNA |
| pLKO.1-TSC2 (shRNA) |
Addgene |
Cat# 15478 |
| NF1 (Myc-DDK-tagged)-Human neurofibromin 1 (NF1), transcript variant 1 |
OriGene Technologies |
Cat# RC220425 |
| pDONR223_PIK3CA_WT |
Addgene |
Cat# 81736 |
| pDONR223_PIK3CA_p.H1047R |
Addgene |
Cat# 82824 |
| pDONR223_KRAS_WT |
Addgene |
Cat# 81923 |
| pDONR223_KRAS_GV12 |
Addgene |
Cat# 31200 |
| Lenti-ORF clone of RASA2 (Myc-DDK-tagged)-Human RAS p21 protein activator 2 (RASA2) |
OriGene Technologies |
Cat# RC224076L3 |
|
| Software and Algorithms |
| Compusyn software |
PMID:20068163 |
N/A |
|
