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. 2020 Jun 18;27:43. doi: 10.1051/parasite/2020041

Table 1.

Rearing schedule with the main tasks and time used in this experiment.

Days Main tasks and time
Day −1 (Monday)
  • Brush eggs

  • Sample (100–150 eggs/sample) and hatch (2 PM)

  • Prepare jars with boiled osmosis water

Day 0 (Tuesday)
  • Determine the egg hatch rate

  • Weigh eggs

  • Hatch eggs in jars (2 PM)

  • Prepare trays with 5 L of osmosis water

Day 1 (Wednesday)
  • Transfer the content of hatching jars (L1) to mass-rearing trays (9 AM)

  • Feed larvae (9 AM)

Day 2 (Thursday)
  • Feed larvae (9 AM)

Day 3 (Friday)
  • Feed larvae (9 AM)

Day 4 (Saturday)
  • Feed larvae (9 AM)

Day 5 (Sunday)
  • Feed larvae (9 AM)

Day 6 (Monday) – Day 9 (Thursday)
  • Tilt the trays (9 AM)

  • Sort larvae and pupae (10–12 AM)

  • Estimate the number of pupae by sex

  • Check female contamination rate

  • Pour the larvae back into the trays and feed (12 AM)

Day 10 (Friday)
  • Tilt the trays (9 AM)

  • Sort larvae and pupae (10–12 AM)

  • Estimate the number of pupae by sex

  • Check female contamination rate