Skip to main content
. Author manuscript; available in PMC: 2020 Nov 8.
Published in final edited form as: ACS Infect Dis. 2019 Sep 26;5(11):1926–1935. doi: 10.1021/acsinfecdis.9b00273

Figure 5: Trypsin digestion patterns of whole E. coli ΔAcrAB(Pore) cells producing the indicated AcrA variants.

Figure 5:

For each reaction, 1.8 × 108 cells producing the wild type AcrA from the chromosome (WT) or the plasmid (WT(plasmid)) and the plasmid-borne AcrA with indicated double cysteine substitutions were treated with trypsin at the final concentrations of 0.0, 0.1, 1.0 and 10.0 μg/ml. Total proteins were separated by 12% SDS-PAGE and probed with polyclonal anti-AcrA antibodies. The major proteolytic fragments of AcrA assembled into the complex, Q29-K374 and T47-R315, are indicated by arrows.