Figure 1.

SARS-CoV-2 nucleocapsid (N) gene sequences and RT-LAMP primer designing. A. The sequences were downloaded from GISAID, the alignment of available sequences was done to design the primer sets which targets the N gene. LAMP primers F3, F2, F1, B1, B2, B3 and loop primers LF and LB locations were highlighted as shown. B. LAMP primer sets designed from the target sequences from position 28,285 to 28,529 have a Forward Inner Primer (FIP) and Backward Inner Primer (BIP), both containing sequence complementary (F1c, B1c) to F2 and B2, respectively. C. RT-LAMP optimized using the final selected primer set. The description and sources for each sequence have been indicated in the acknowledgment section. The positive RT-LAMP amplification was optimized using the Primer set enlisted in Table 1. Lane M: 100 bp DNA ladder; Lane N: negative control, lane P: results from the RT-LAMP amplification.