Figure 4.

Determination of specificity of the RT-LAMP for SARS-CoV-2. RT-LAMP was performed against panels of A. related coronaviruses, such as human coronaviruses (OC43, NL63, and 229E), and MERS-CoV, B. human infectious influenza viruses, including highly pathogenic avian influenza viruses, and C. avian influenza viruses (low pathogenic). Each set of the panel includes Upper: RT-LAMP, Middle: RT-LAMP products electrophoresed and Lower: One-step RT-PCR Viral RNA confirmation of the samples used in the experiment. Specific primers used for confirmation of amplification of each viral RNA used from various respiratory disease-causing viruses have been indicated in Supplementary Table 2. MERS: Middle East respiratory syndrome coronavirus; B-Y: B/Phuket/3073/2013 (Yamagata lineage); B-V: B/Brisbane/60/2008 (Victoria lineage). Lane M: 1000 bp DNA ladder; PC: SARS-CoV-2 viral RNA (1ng/reaction); Lane N.C: negative control