TABLE 1.

Target genes, primer list and amplification protocol (mouse).

Gene	NCBI Accession No.	Sequences
m18S	NR_003278.3	Forward	ACTCAACACGGGAAACCTCACC
		Reverse	CCAGACAAATCGCTCCACCA
mGlut1	NM_011400.3	Forward	TGGCGGGAGACGCATAGTTA
		Reverse	CTCCCACAGCCAACATGAGG
mGlut3	NM_011401.4	Forward	GAGGAACACTTGCTGCCGAG
		Reverse	CTGGAAAGAGCCGATCGTGG
mGlut4	NM_009204.2	Forward	CTTATTGCAGCGCCTGAGTC
		Reverse	GGGTTCCCCATCGTCAGAG
mMCT1	NM_009196.4	Forward	AGTGCAACGACCAGTGAAG
		Reverse	GCGATCATTACTGGACGGCT
mMCT2	NM_001358496.1	Forward	CAGCAACAGCGTGATAGAGC
		Reverse	AGGCTGGTTGCAGGTTGAAT
mMCT4	NM_001038653.1	Forward	GGCTGGCGGTAACAGAGTA
		Reverse	CGGCCTCGGACCTGAGTATT
mNa+/K+-ATPaseα1	NM_144900.2	Forward	TATCCTACTGCCCCGGGATG
		Reverse	CTTCCGCACCTCGTCATACA
mNa+/K+-ATPaseα2	NM_178405.3	Forward	TTCGCTAGCATCGTGGTTGT
		Reverse	CAGCCAAAGCCGTCTCTTCT
mNa+/K+-ATPaseα3	NM_001290469.1	Forward	TTGCCAAGGGTGTGGGTATC
		Reverse	GGTGAAGTCCTTGAGGTCGG
mHif1α	NM_001313919.1	Forward	AGCCAGCAAGTCCTTCTGAT
		Reverse	AGGCTGGGAAAAGTTAGGAGTG
mPHD3	NM_028133.2	Forward	ATCCACATGAAGTCCAGCCC
		Reverse	ACACCACAGTCAGTCTTTAGCA
mPDK1	NM_172665.5	Forward	TGCAAAGTTGGTATATCCAAAGCC
		Reverse	TGTGCCGGTTTCTGATCCTT
Segment	Plateau	Temperature	Duration	Cycle
Hot Start	1	50	0:03:00	1
Hot Start 2	1	95	0:03:00	1
Amplification	1	95	0:00:05	40
Amplification	2	60	0:00:30	40
Melt	1	95	0:00:30	1
Melt	2	65	0:00:30	1
Melt	3	95	0:00:30	1