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. 2020 May 20;9(1):1011–1022. doi: 10.1080/22221751.2020.1763209

Figure 2.

Figure 2.

Conjugation frequencies of p187-2 in BW25113 strains with or without KP8 CRISPR. (A) (i) Schematic of JS681 and JS683. (ii)Expression of the Cas operon in the KP8, JS683 and JS681 cells. (B) Effect of the KP8 CRISPR on the conjugation frequencies of p187-2(IncF conjugative plasmid with blaKPC and matched proto-spacers). The data represent the mean ± SD for six independent biological replicates. p = 0.0008 indicate significant differences between two groups as determined using two-tailed Student’s t-test.