Figure 3.

YYWY exerted anti-tumor effects through enhancing the immune response in Lewis-bearing mice. (A, B) KEGG pathway and GO enrichment analysis for the up-regulated genes of the tissue tumor in YYWY treated Lewis-bearing mice vs. controls (n = 3). (C) Immunofluorescence of the CD11c (red) and CD8 (green) combined with DAPI staining (blue) in tumor tissue from Lewis-bearing mice. Scale bar: 50 μm. (D) Representative immunohistochemical staining of CD4 and CD8 T cells were carried out in the tumor tissue from Lewis-bearing mice. Scale bar: 100μm. (E) Production of the DCs by spleen stimulated with YYWY or 0.9% normal saline (control) for 14 day as determined by flow cytometry. (F) Proliferation of CD4⁺ and CD8⁺ T cell in spleen stimulated with YYWY and 0.9% normal saline. The values represent the mean ± SEM of eight independent experiments. ***p < 0.001 compared to the control group. RNA-SEQ was performed in triplicate on the tumor tissues from Lewis-bearing mice and the controls.