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. 2020 Jun 9;11:798. doi: 10.3389/fphar.2020.00798

Figure 4.

Figure 4

YYWY promoted the maturation of DCs. (A) BMDCs was treated with YYWY or LPS for 24 h. The cytotoxicity of YYWY on BMDCs was determined by CCK-8 assay. (B) Analysis of apoptosis by Annexin V/PI double-staining assay of BMDCs after YYWY (50 or 100 μg/ml) or LPS (1 μg/ml) treatment for 24 h, and the representative FACS analysis scatter grams of Annexin V-FITC/PI staining are presented. (C) BMDCs were treated with YYWY (50 or 100 μg/ml) for 24 h in the presence or absence of a pre-treatment of TLR4 blocker TAK-242 for 24 h, then DCs maturation markers (CD11c, MHCII, CD40/83/86) was detected by flow cytometry. Representative histograms displaying levels of fluorescent CD80, CD83, and CD11c are presented. The percentage of CD80+ and CD83+ cells in total CD11c+ cells are presented on the below. (D) The ultrastructure of BMDCs after YYWY treatment were observed through TEM. Scale bar: 5μM. (E) The mRNA levels of DCs-secreted cytokines (IFN-γ, IL-1β, TNF-α, IL-2, and IL-12) were detected via qPCR. The values represent the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the control group.