Fig. 1.

Inhibition of PEDV replication by A77 1726. (A) IPEC-DQ cells were infected with the HLJBY strain of PEDV (0.5 MOI) and then incubated in the absence or presence of the indicated concentration of A77 1726 without or with uridine (200 μM) for 24 h. The cell lysates were analyzed for the expression of the S and N proteins by Western blot. Vero cells were infected with HXLV (B & D) or CAJ strain (C & F) of PEDV, 0.5 MOI each, were incubated in the absence or presence of the indicated concentration of A77 1726 without or with uridine (200 μM) for 12 h. The cell lysates were analyzed for the expression of the S and N proteins by Western blot. (D & F) The conditioned media were collected and analyzed for the TCID₅₀ values. The results represent the mean ± standard deviation (SD) of three independent experiments and statistically analyzed by a Student's t-test. *p < 0.05, **p < 0.01. (E & G) Effect of A77 1726 (E) and uridine (G) on Vero cell proliferation. Vero cells seeded in 96-well plates were left uninfected or infected with PEDV virus (0.5 MOI) and then incubated in the absence or presence of A77 1726 or uridine (200 μM each). After incubation for 12 h, the cells were analyzed for proliferation by using a CellTiter-Glo kit. Data are the mean ± SD of the triplicate from one representative of three independent experiments with similar results.