Fig. 6.

Overexpression of JAK2, STAT3, and Src enhances PEDV replication and attenuates the antiviral activity of A77 1726. Vero cells were transfected with the pcDNA3.1 empty expression vector or the vector encoding JAK2 (A), STAT3 (B), or a constitutively active Src gene (C). After incubation for 36 h, the cells were infected with HXLV (0.5 MOI) and then incubated in the absence or presence of the indicated concentrations of A77 1726 for 12 h. The cell lysates were analyzed for the levels of the S and N proteins or tyrosine phosphorylation of JAK2, STAT3, and Src by Western blot. The density of the S and N proteins and the phosphorylated JAK2, STAT3, and Src bands was analyzed by using NIH Image-J software and normalized by the arbitrary units of β-actin or their corresponding total proteins. *p < 0.05, **p < 0.01, compared to the corresponding samples in the cells transfected with pcDNA3.1 with a Student's t-test. (D-F) The conditioned media were collected and analyzed for the TCID₅₀ values. The results represent the mean ± standard deviation (SD) of three independent experiments and statistically analyzed by a Student's t-test. *p < 0.05, **p < 0.01.