TABLE 1.
Fermentation products of the mutants analyzed in this studya
| Strain or genotype | Product (g/liter) |
Glucose consumption (g/liter) | Final pH | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Solvents |
Acids |
|||||||||
| Ethanol | Acetone | Butanol | Total | Acetate | Butyrate | Lactate | Total | |||
| WT | 1.3 ± 0.1 | 9.0 ± 0.5 | 12.4 ± 1.4 | 22.8 ± 2.0 | 2.6 ± 0.1 | 2.4 ± 0.1 | NDb | 5.0 ± 0.2 | 69.2 ± 0.2 | 5.1 ± 0.0 |
| ΔbdhA | 1.2 ± 0.1 | 9.1 ± 0.4 | 11.6 ± 0.4 | 22.0 ± 0.1 | 2.4 ± 0.1 | 2.3 ± 0.1 | ND | 4.7 ± 0.0 | 69.4 ± 0.2 | 5.2 ± 0.0 |
| ΔbdhB | 0.9 ± 0.0 | 9.2 ± 0.2 | 12.1 ± 0.6 | 22.2 ± 0.6 | 2.2 ± 0.1 | 2.7 ± 0.1 | ND | 4.9 ± 0.2 | 71.0 ± 2.4 | 5.3 ± 0.0 |
| ΔbdhC | 1.3 ± 0.2 | 9.3 ± 0.3 | 12.8 ± 1.0 | 23.4 ± 1.4 | 2.6 ± 0.4 | 2.1 ± 0.5 | ND | 4.7 ± 0.9 | 71.4 ± 2.6 | 5.3 ± 0.1 |
| ΔbdhA ΔbdhB | 0.5 ± 0.0 | 3.8 ± 0.6 | 3.2 ± 0.7 | 7.4 ± 1.4 | 4.5 ± 0.4 | 5.0 ± 0.2 | 0.2 ± 0.1 | 9.7 ± 0.4 | 35.9 ± 2.2 | 4.8 ± 0.0 |
| ΔbdhA ΔbdhC | 1.1 ± 0.2 | 9.5 ± 0.5 | 13.3 ± 0.1 | 23.9 ± 0.4 | 2.2 ± 0.2 | 2.8 ± 0.4 | ND | 5.0 ± 0.2 | 72.0 ± 0.6 | 5.2 ± 0.0 |
| ΔbdhB ΔbdhC | 1.0 ± 0.1 | 9.3 ± 1.1 | 11.6 ± 0.7 | 21.9 ± 1.6 | 2.0 ± 0.2 | 2.9 ± 0.5 | ND | 4.9 ± 0.2 | 73.5 ± 1.8 | 5.3 ± 0.0 |
| ΔbdhA ΔbdhB ΔbdhC | 0.4 ± 0.0 | 2.5 ± 0.2 | 1.3 ± 0.2 | 4.2 ± 0.3 | 5.3 ± 0.3 | 5.9 ± 0.2 | 0.2 ± 0.1 | 11.4 ± 0.5 | 26.7 ± 1.8 | 4.7 ± 0.0 |
a
Results shown are average values ± standard deviations from at least three independent technical replicates. For mutant assays, values are from biological replicates.
b
ND, not detected.