TABLE 3.

Complementation assays^a

Strain or geneotype	Plasmid	Products (g/liter)								Glucose consumption (g/liter)	Final pH
		Solvents				Acids
		Ethanol	Acetone	Butanol	Total	Acetate	Butyrate	Lactate	Total
WT	pFW01	1.8 ± 0.1	8.9 ± 0.4	13.3 ± 0.7	24.0 ± 1.1	2.2 ± 0.2	2.5 ± 0.1	NDb	4.7 ± 0.2	64.0 ± 1.3	5.3 ± 0.1
	pFW01-bdhA	2.2 ± 0.1	9.9 ± 0.2	11.9 ± 0.6	24.0 ± 0.9	1.7 ± 0.3	1.4 ± 0.4	ND	3.2 ± 0.7	64.8 ± 1.5	5.3 ± 0.1
	pFW01-bdhB	1.6 ± 0.3	8.4 ± 1.1	12.1 ± 1.3	22.1 ± 2.6	2.3 ± 0.3	2.7 ± 0.2	ND	5.0 ± 0.5	61.5 ± 2.5	5.1 ± 0.0
	pFW01-bdhC	1.8 ± 0.4	9.6 ± 0.5	13.4 ± 0.7	24.8 ± 1.5	2.4 ± 0.1	2.3 ± 0.1	ND	4.7 ± 0.2	64.2 ± 0.6	5.2 ± 0.0
ΔbdhA ΔbdhB	pFW01	0.7 ± 0.0	2.6 ± 0.7	2.7 ± 0.8	6.1 ± 1.5	5.4 ± 0.4	4.1 ± 0.6	0.6 ± 0.1	10.1 ± 1.0	21.4 ± 3.4	4.7 ± 0.1
	pFW01-bdhA	2.0 ± 0.1	10.6 ± 1.1	13.7 ± 0.8	26.3 ± 0.9	1.7 ± 0.5	1.3 ± 0.1	ND	3.0 ± 0.5	70.9 ± 2.2	5.5 ± 0.1
	pFW01-bdhB	1.8 ± 0.1	8.8 ± 0.5	13.6 ± 0.1	24.2 ± 0.3	2.1 ± 0.4	1.6 ± 0.5	ND	3.7 ± 0.9	68.7 ± 0.6	5.4 ± 0.3
	pFW01-bdhC	1.4 ± 0.1	10.2 ± 0.4	13.2 ± 0.7	24.8 ± 1.2	1.9 ± 0.1	2.5 ± 0.1	ND	4.4 ± 0.2	67.7 ± 1.0	5.4 ± 0.0
ΔbdhA ΔbdhB ΔbdhC	pFW01	0.5 ± 0.1	2.3 ± 0.3	2.1 ± 0.3	4.9 ± 0.6	5.7 ± 0.5	4.6 ± 0.4	0.6 ± 0.1	10.9 ± 1.0	18.8 ± 3.4	4.6 ± 0.0
	pFW01-bdhA	1.4 ± 0.1	9.6 ± 0.3	13.2 ± 0.3	24.2 ± 0.6	1.9 ± 0.0	1.8 ± 0.1	ND	3.7 ± 0.2	68.6 ± 1.1	5.4 ± 0.1
	pFW01-bdhB	1.7 ± 0.2	8.7 ± 0.8	13.8 ± 0.7	24.2 ± 1.7	1.9 ± 0.0	1.7 ± 0.1	ND	3.6 ± 0.1	68.6 ± 1.2	5.5 ± 0.1
	pFW01-bdhC	1.2 ± 0.3	10.4 ± 0.8	12.3 ± 0.5	23.9 ± 0.7	1.8 ± 0.4	2.1 ± 1.1	ND	3.9 ± 1.5	67.4 ± 0.4	5.4 ± 0.1

^aResults shown are average values ± standard deviations from at least three independent biological replicates.

^bND, not detected.