FIG 5.
Utilizing qPCR to determine fimbria mutant fitness during UTI. Ten BALB/c mice were transurethrally inoculated with 108 CFU of mixed CFT073 mutants. Twenty-three unique gene transposon mutants with disruptions in known fimbria genes were selected to compete with the marked wild-type ΔaraF::Kan strain in co-challenge. qPCR on the gDNA harvested from the mouse bladders was used to determine log10 CI compared to the total bacterial gDNA. Ordered library mutants have been categorized by the domain containing the insertion: major, minor, usher, adhesion, and hypothetical components. The type 1 fimbrial gene cluster has 3 represented mutants (30 total symbols), F1C has 4, Auf has 3, Yad has 4, Ygi is represented by a single transposon mutant, Yeh has 2, Mat has 3, and Yfc has 3. P values determined by the Wilcoxon signed-rank test are listed if significant.