FIG 6.

Applying the qPCR co-challenge technique to groups of toxin mutants from bladder and kidneys. Fourteen BALB/c mice were transurethrally inoculated with 10⁸ CFU of mixed CFT073 mutants. We combined 13 transposon mutants, pulled from the ordered library, with mutations in known toxins and virulence genes and ranked these in relation to wild-type CFT073. Each mouse is represented by a single color; the black line indicates the median. qPCR was performed on the gDNA harvested from mouse bladders (A) or kidneys (B) to determine Log₁₀ CI compared to WT. If significant, P values determined by the Wilcoxon signed-rank test are listed.