FIG 7.

Co-challenge validation of select genes discovered using human urine in vitro Tn-seq. Cocultures of 1:100 back diluted wild-type CFT073 and a single isogenic transposon mutant were incubated statically at 37°C in human urine for 24 h. Differential plating for CFU was done at 0, 2, 4, 6, 8, 10, and 24 h. The mean from three independent trials is plotted by a single dot at every time point, displayed with standard error of the mean (SEM). Each mutant is individually plotted with wild-type CFT073 for comparison.