TABLE 1.
E. coli CFT073 genes tested in the UTI model showing significant fitness defects (≥513-fold change; P < 0.05) for colonization of the murine bladder
| Locus |
Protein | Fold change | P value | Adjusted P value | |
|---|---|---|---|---|---|
| RefSeq | GenBank | ||||
| C_RS19445 | c4105_pshM | Putative general secretion pathway protein M (Transport protein PshM) | 2,737.2 | 0.0211 | 0.5358 |
| C_RS25485 | c5399_fimG | FimG protein precursor | 2,032.6 | 0.0269 | 0.5711 |
| C_RS24715 | c5232_efp | Elongation factor P | 1,623.2 | 0.0296 | 0.5711 |
| C_RS22205 | c4704_rfe | Undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase | 1,583.6 | 0.0005 | 0.2269 |
| C_RS00190 | c0041_carB | Carbamoyl-phosphate synthase large chain | 1,456.1 | 0.0159 | 0.5042 |
| C_RS21030 | c4456_rfaQ | Lipopolysaccharide core biosynthesis glycosyl transferase RfaQ | 1,380.3 | 0.0167 | 0.5042 |
| C_RS23585 | c4979_yjbB | Hypothetical protein YjbB | 1,346.3 | 0.0287 | 0.5711 |
| C_RS25490 | c5400_fimH | FimH protein precursor | 1,340.6 | 0.0384 | 0.6299 |
| C_RS10865 | c2294_flhB | Flagellar biosynthetic protein FlhB | 1,340.3 | 0.0172 | 0.5042 |
| C_RS24925 | c5277_yjfO | Hypothetical lipoprotein YjfO precursor | 1,185.5 | 0.0193 | 0.5291 |
| C_RS07675 | c1641_ldcA | Muramoyltetrapeptide carboxypeptidase | 1,174.8 | 0.0011 | 0.2269 |
| C_RS10925 | c2308_flhD | Flagellar transcriptional activator FlhD | 1,126.5 | 0.0203 | 0.5304 |
| C_RS22705 | c4803_yihE | Hypothetical protein YihE | 1,110.5 | 0.0164 | 0.5042 |
| C_RS11185 | c2361_fliL | Flagellar FliL protein | 1,061.3 | 0.0012 | 0.2269 |
| C_RS14420 | c3035_yfgM | Hypothetical protein YfgM | 1,052.3 | 0.0038 | 0.3253 |
| C_RS06310 | c1345_flgE | Flagellar hook protein FlgE | 1,023.6 | 0.0012 | 0.2269 |
| C_RS27145 | c2497 | Transposase | 947.5 | 0.0240 | 0.5711 |
| C_RS17605 | c3712 | Putative saframycin Mx1 synthetase B | 938.1 | 0.0013 | 0.2269 |
| C_RS06175 | c1314_mdoH | Periplasmic glucans biosynthesis protein MdoH | 937.4 | 0.0024 | 0.3082 |
| C_RS21025 | c4455_rfaG | Lipopolysaccharide core biosynthesis protein RfaG (glucosyltransferase I) | 923.7 | 0.0017 | 0.2625 |
| C_RS08200 | c1750_pyrF | Orotidine 5′-phosphate decarboxylase | 903.9 | 0.0250 | 0.5711 |
| C_RS22120 | c4687_yifB | Hypothetical protein YifB (ORF III) | 751.3 | 0.0057 | 0.3493 |
| C_RS19985 | c4222 | Putative DNA-processing protein | 746.8 | 0.0012 | 0.2269 |
| C_RS07045 | c1510_purB | Adenylosuccinate lyase | 738.2 | 0.0304 | 0.5772 |
| C_RS18670 | c3936_ftsJ | Ribosomal RNA large subunit methyltransferase J | 717.2 | 0.0067 | 0.3584 |
| C_RS03055 | c0660_cusB | Putative copper efflux system protein CusB precursor | 707.7 | 0.0011 | 0.2269 |
| C_RS23265 | c4922_oxyR | Hydrogen peroxide-inducible genes activator (morphology and autoaggregation control protein) | 707.3 | 0.0271 | 0.5711 |
| C_RS24085 | c5093_yjcW | d-Allose transport ATP-binding protein AlsA | 671.0 | 0.0365 | 0.6258 |
| C_RS06240 | c1329_pyrC | Dihydroorotase | 652.1 | 0.0026 | 0.3118 |
| C_RS11080 | c2338_fliC | Flagellin | 551.4 | 0.0026 | 0.3118 |
| C_RS22910 | c4847_fdhD | FdhD protein | 541.0 | 0.0356 | 0.6233 |
| C_RS14270 | c3004_purC | Phosphoribosylaminoimidazole-succinocarboxamide synthase | 531.0 | 0.0031 | 0.3253 |
| C_RS02790 | c0606_ybaT | Hypothetical transport protein YbaT | 530.6 | 0.0067 | 0.3584 |
| C_RS08560 | c1822_ompN | Outer membrane protein N precursor (porin OmpN) | 522.5 | 0.0248 | 0.5711 |
| C_RS16250 | c3425_ygdP | (Di)nucleoside polyphosphate hydrolase | 518.6 | 0.0094 | 0.4128 |
| C_RS11255 | c2378_yedA | Hypothetical transport protein YedA | 513.9 | 0.0230 | 0.5520 |