Fitness scores for select E. coli genes. (A to C) Mean fitness scores for select genes involved in the NSR (A), global stress responses (B), and de novo nucleotide biosynthesis (C) for E. coli grown in monoculture (gray), Nx coculture (teal), or NxΔAmtB coculture (yellow). Error bars indicate standard deviations (n = 4). In panel C, asterisks (*) denote genes known to have redundant functions or known to be dispensable for de novo synthesis.