FIG 7.

Influence of CrhB on the catalytic activity of AnaRne in vitro. (A) Enzyme activity assays of AnaRne in the presence or absence of CrhB. +, presence of protein(s) or RNA; −, absence of protein(s) or RNA. (B) Unwinding activity of CrhB on 5′ dsRNA. CrhB concentrations were adjusted as indicated. (C) The RNA unwinding activity of CrhB depends on ATP. ATP concentrations in the reaction are shown above the lanes. (D) The RNA unwinding activity of CrhB is Mg²⁺ dependent. The Mg²⁺ concentrations are shown above the lanes. The RNA sequences are described in Materials and Methods. Data correspond to results from three independent experiments.