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. 2020 May 11;2(6):344–356. doi: 10.1002/acr2.11127

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© 2020 The Authors. ACR Open Rheumatology published by Wiley Periodicals, Inc. on behalf of American College of Rheumatology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunoglobulin G1 (IgG1) antibodies against phosphorylcholine (anti‐PCs) inhibit interleukin 6 (IL‐6) production by lipopolysaccharide (LPS)‐stimulated THP1‐like macrophages. Anti‐PC clones reduce IL‐6 production on LPS‐stimulated THP1 cells. THP1 monocytes were stimulated with 100 ng/ml phorbol 12‐myristate 13‐acetate for 72 hours. The cells were preincubated with IgG1 anti‐PCs at different concentrations (100 ng/ml to 20 μg/ml) prior to LPS (100 ng/ml) stimulation overnight. Supernatants were collected the next day and analyzed for IL‐6 secretion. All three antibodies reduced IL‐6 production even at a low concentration, such as 100 ng/ml. The graphs represent the mean ± SD of three independent experiments. A, A01. B, D05. C, E01. D, Isotype control. NS, non‐significant.