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. Author manuscript; available in PMC: 2020 Jun 18.
Published in final edited form as: Cell. 2019 Jun 13;177(7):1771–1780.e12. doi: 10.1016/j.cell.2019.05.032

Figure 3. Cryo-electron Tomography Revealing Capsids Trapped along Mutant PhuZ Filaments during Phage 𝚽PA3 Infection in P. aeruginosa at 70 mpi.

Figure 3.

(A) A slice through a tomogram of a cryo-focused ion beam–thinned phage-infected cell at 70 mpi. Scale bar, 200 nm.

(B) Annotation of the tomogram in (A) showing extracted structures, including capsids (green), cytoplasmic membrane (pink), outer membrane (red), and mutant PhuZD190A spindles (blue). Scale bar, 200 nm.

(C and D) Zoomed-in view (C) of one of the capsids stuck on the mutant filament from the tomogram shown in (A) and its corresponding tomogram slice (D). Scale bar, 50 nm.

(E–H) Slices of tomograms of capsids trapped along the mutant filaments from tomograms of other phage-infected cells taken for this study, with blue arrow pointing towards the mutant spindle.

See also Figure S2.