REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Bacterial and Virus Strains | ||
NEB 5-alpha Competent E. coli | New England Biolabs | Cat#C2987H |
Pseudomonas aeruginosa (PAO1) | Pogliano strain collection | N/A |
Pseudomonas aeruginosa K2733 | Keith Poole of Queen’s University Kingston, ON, Canada |
N/A |
Pseudomonas chlororaphis 200-B | Drs. Julie Thomas and Steven Hardies of UT Health Sciences San Antonio | N/A |
Pseudomonas aeruginosa phage PhiPA3 | Dr. Marcy Erb | N/A |
Pseudomonas chlororaphis phage 201phi2–1 | Drs. Julie Thomas and Steven Hardies of UT Health Sciences San Antonio | N/A |
Saccharomyces cerevisiae Y2H Gold | Clontech | Cat#630489 |
Saccharomyces cerevisiae Y187 | Clontech | Cat#630489 |
See Table S1 for a complete list of strains used in this paper | N/A | |
Chemicals, Peptides, and Recombinant Proteins | ||
FM 4–64 | Thermo Fisher Scientific | Cat#T13320 |
DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) |
Thermo Fisher Scientific | Cat#D1306 |
Ticarcillin disodium salt | Sigma-Aldrich | Cat#T5639 |
Gentamycin sulfate | Sigma-Aldrich | Cat#G1914 |
Phusion High-Fidelity DNA Polymerase | New England Biolabs | Cat#M0530L |
Deoxynucleotide (dNTP) Solution Mix | New England Biolabs | Cat#N0447L |
NEBuilder HiFi DNA Assembly Cloning Kit | New England Biolabs | Cat#E5520S |
T4 DNA ligase | New England Biolabs | Cat#M0202L |
Nucleospin plasmid extraction kit | Macherey-Nagel | Cat#740588 |
NucleoSpin Gel and PCR Clean-up | Macherey-Nagel | Cat#740609 |
Matchmaker Gold Yeast Two-Hybrid System | Clontech | Cat#630489 |
Oligonucleotides | ||
See Table S2 for a complete list of oligonucleotides used in this paper | N/A | |
Recombinant DNA | ||
pHERD30T plasmid | Dr. Hongwei D Yu of Marshall University | N/A |
See Tables S3 and S4 | N/A | |
Software and Algorithms | ||
DeltaVision SoftWoRx 6.5.2 | GE HealthCare | http://incelldownload.gehealthcare.com/bin/download_data/SoftWoRx/7.0.0/SoftWoRx.htm |
FIJI/ImageJ | NIH | https://imagej.nih.gov/ij/ |
CellProfiler 2.0 | Lamprecht et al., 2007 | cellprofiler.org |
Amira | Commercial software by Thermo Scientific (formerly FEI) |
https://www.fei.com/software/amira-3d-for-lifesciences/; RRID:SCR_014305 |
TomoSegMemTV | Martinez-Sanchez et al., 2014 | https://sites.google.com/site/3demimageprocessing/tomosegmemtv |
IMOD | Kremer et al., 1996 | http://bio3d.colorado.edu/imod/; RRID:SCR_003297 |
Microsoft Excel | Microsoft Office | https://products.office.com/en-us/excel |
Adobe Photoshop | Adobe | https://www.adobe.com/products/photoshop.html |
Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
Adobe Acrobat | Adobe | https://www.adobe.com/products/acrobat.html |