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. 2020 Jun 17;20:253. doi: 10.1186/s12935-020-01337-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Effects of individual and combination treatments of ribociclib and adavosertib on spheroid growth and composition. a Representative images of time course spheroid growth of spheroids of different compositions. Images present the merged images of the Venus and mCherry channels. White bars correspond to 1000 µm. b Time course growth of spheroids presented on Panel A. Data points show average spheroid areas of three replicates, error bars show standard deviation if it is larger than the size of the data point. Vertical lines represent statistical comparisons of the spheroid area between spheroids on Day 21. Asterisks mark statistical significance (*p < 0.05, **p < 0.01). Further statistical analysis including all time points and raw data are presented in Additional file 1: Table S6. c Representative FACS analysis of mixed spheroids on Day 21 on untreated, ribociclib, adavosertib and ribociclib + adavosertib treated spheroids initiated in a composition of 50% sensitive and 50% resistant cells. Samples are identical to the mixed samples on Panel A (second, fifth, eighth and eleventh rows). d Proportion of sensitive cells under different treatments based on FACS analysis. Bars show the average of three replicates, error bars show standard deviation. Asterisks mark statistical significance (p < 0.05)