Table 2.
Human spermatogenesis single-cell RNA-seq datasets.
| Guo J et al. (2017), Cell Stem Cell | Guo J et al. (2018), Cell Res | Hermann BP et al. (2018), Cell Rep | Neuhaus N et al. (2017) Mol Hum Reprod | Sohni A et al. (2019), Cell Rep | Wang M et al. (2018), Cell Stem Cell | |
|---|---|---|---|---|---|---|
| Total cell number | 92 (from 1 to 5 men) | ∼7792 (6492 from 2 of 17-years-old, 24-years-old, and 25-years-old and ∼1300 from 12 to 13 month) | 37 086 (from 15 men) | 105 (from 1 patients with spermatogonial arrest, from 7 normal) | 33 585 (18 723 from 2 adult men and 14 862 from 2 neonates) | 3028 (2854 from normal or OA and 174 from NOA) |
| Young spermatogonia | – | + | – | – | + | – |
| Unselected steady-state spermtogenesis | – | + | + | – | + | + |
| Sorted Spermatogonia | + | + | + | + | + | + |
| Sorted Spermatocytes | – | + | + | – | + | + |
| Sorted Spermatids | – | + | + | – | + | + |
| Sperm | – | + | – | – | – | – |
| Somatic cells | – | Macrophages, Sertoli cells, Leydig cells, Myoid cells, Endothelial cells | Sertoli cells, Leydig cells, Peritubular cells, Macrophage, Perivascular cells (adult) | – | Sertoli cells, pertibular myoid cells, blood/endothelial cells, macrophages, Leydig cells (adult) Peritubular myoid cells, Leydig cells, blood/endothelial cells (neonatal) | Sertoli cells, peritubular myoid cells, Leydig cells, testicular macrophages (normal or OA and NOA patiants) |
| Validation methods | IHC | smFISH | IHC | IHC | IHC, Flow cytometory | IHC, ISH, NOA |
| scRNA-seq Chemistry/Method | full length sequence (Fluidigm C1) | 3′ sequencing and barcoding (10x Genomics) | full length sequence (Fluidigm C1), 3′ sequencing and barcoding (10x Genomics) | full length sequence | 3′ sequencing and barcoding (10x Genomics) | 3′ sequencing (SMART-seq2) |
| Replication | Replication unclear | 3 biological replicates from healthy men and 2 replicates from infants (each with 2 techinical replicates) | 9 x C1 adult human spermatogonia, 3 x 10x Genomics adult steady-state spermatogenesis (unselected), 2 x Adult StaPut Spermatocytes, 2 x Adult StaPut Round Spermatids | 2 biological replicates | One replicate each from neonatal day 2 ITGA6 enriched or unfractionated and neonatal day 7 ITGA6 enriched or unfractionated, 2 adult replicates, each with ITGA6 enriched and unfractionated. | 2 replicates from healthy men with obstructive azoospermia, 1 replicate from man with nonobstructive azoospermia (NOA) |
| GEO number | GSE92280 | GSE120508 | GSE108970, GSE108974, GSE108977, GSE109049, GSE109033, and GSE109037 | None | GSE124263 | GSE106487 |