Fig. 3.

Mig-6 regulates the proliferation and migration of lung adenocarcinoma cells. a Western blot analysis of Mig-6 in PC/GR cells following transfection with Mig-6 siRNA or scrambled siRNA. b Cell viability was assessed using the CCK-8 assay after transfection of PC9 and PC9/GR cells with Mig-6 siRNA or scrambled siRNA. c Cell migration ability was tested using wound-healing and transwell migration assays. PC9 and PC9/GR cells were transfected with vector or siMig-6. Movement of cells into wounds is shown at 0 and 24 h post-scratching. Scale bar: 200 mm. Data are shown as the mean ± standard deviation (SD); *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test. d Western blot analysis of E-cadherin and Vimentin in PC9 and PC9/GR cells with Mig-6 siRNA or scrambled siRNA. e Western blot analysis of AKT and p-AKT in PC9 and PC9/GR cells with Mig-6 siRNA or scrambled siRNA. f Western blot analysis of EGFR, p-EGFR (Tyr1068), p-EGFR (Tyr1045), ERK and p-ERK in PC9 and PC9/GR cells with Mig-6 siRNA or scrambled siRNA. β-actin was used as a loading control. Uncropped blots were shown in Additional file 1: Fig. S3