Figure 5.

Time course of effect of Ab181 (at 10 mg/kg) on a maxadilan-induced increase in DBF in rats. Rats were given intravenous bolus injection of Ab181 at 10 mg/kg with pretreatment time at 0.5, 3, 6.25, 48, and 168 hours before maxadilan challenge (n = 3-8 rats/group). After obtaining a postdrug baseline (BL), maxadilan at a dose of 10 ng (in 20 µL DPBS) was injected intradermally. The DBF was measured by a laser Doppler imager. Solid triangle represents % inhibition (mean ± SEM, left axis), and open bar represents resulting serum concentration (mean ± SD, right axis). DBF, dermal blood flow; DBPS, Dulbecco's phosphate-buffered saline.